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2023 Vol.3, Issue 2 Preview Page
Isolation and Characterization of Freshwater Microalga Nautococcus sp. KNUA212 and Bioenergy Analysis

국내 담수 미세조류 Nautococcus sp. KNUA212의 분리 및 바이오 에너지 분석

Ha Neul Choi1
,
Jeong-Mi Do12
,
Ho-Sung Yoon123*
최 하늘1
,
도 정미12
,
윤 호성123*
1Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu 41566, Korea
2BK21 FOUR KNU Creative BioResearch Group, School of Life Sciences, Kyungpook National University, Daegu 41566, Korea
3Advanced Bio-Resource Research Center, Kyungpook National University, Daegu 41566, Korea
1경북대학교 자연과학대학 생명과학부
2경북대학교 첨단복합 생명과학 교육연구단(생명과학부 4단계 BK교육연구단)
3경북대학교 신바이오소재연구소
*Corresponding Author
31 December 2023. pp. 91-102
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Abstract

Increased carbon dioxide in atmosphere by increased use of fossil fuel leads to the climate change, unstable price of fuels and the decline of biodiversity. The microalgae can eliminate the carbon dioxide through photosynthesis and accumulate the lipid that can be used for biofuels as the alternative to these problems. In addition, the highly valued products (pigments, polysaccharides and amino acids etc.) are available for diverse industrial use and for those reasons, many related studies have performed actively. Our team collected sample from Ulleungdo fresh water to perform morphologic and molecular biological species identification and classified the microalgae as Nautococcus sp. KNUA212. As a result of cultivating Nautococcus sp. KNUA212 in BG-11 liquid medium for 8 days while maintaining 1~3% CO2 supply, we could harvest the biomass on the 8th day of which a dry weight was 1.34 g L–1. Biochemical analysis of the freeze-dried biomass showed that Nautococcus sp. KNUA212 had a high protein content of 26.77%. Approximate analysis of the biomass showed that the high volatile matter of 89.35%, which was much higher than the value measured in crops and wood, first and second-generation biofuels. Additionally, elemental analysis of the biomass showed that the high calorific value of 22.0 MJ kg–1, which was higher or similar to that of previous bio and fossil fuel. Especially, the calorific value of Nautococcus sp. KNUA212 was much higher than the values ​​measured in crops and wood, first and second-generation biofuels and we could identify that Nautococcus sp. KNUA212 also can be utilized as the bio energy source such as biofuel.

Keywords
bio energy source
biofuel, biomass
highly valued products
lipid
microalgae

과도한 화석연료 사용으로 증가한 대기중 이산화탄소는 기후 변화와 불안정한 연료 비용, 생물 다양성의 감소문제를 야기했다. 이러한 문제의 대안책으로써 광합성을 통해 대기 중의 이산화탄소를 흡수하고 바이오 연료로 이용가능한 지질을 축적하는 미세조류가 각광받고 있다. 또한 미세조류의 바이오매스에 축적된 고부가가치 산물들(색소, 다당류, 아미노산 등)은 산업적으로 다양하게 활용할 수 있어 관련 연구들이 활발하게 이루어지고 있다. 본 연구는 울릉도 담수에서 채집해 순수분리한 토착 미세조류를 형태학적 및 분자생물학적으로 동정하여 Nautococcus 속으로 분류했다. Nautococcus sp. KNUA212를 BG-11 액체 배지에서 1~3%의 CO2 공급을 유지하며 8일간 배양한 결과 배양 1.34 g L–1의 건조중량을 가지는 바이오매스를 수확할 수 있었다. 동결건조한 바이오매스의 생화학적 특성을 분석한 결과 26.77%의 단백질 함량을 가진 것으로 나타났다. 근사분석을 통해 89.35%의 높은 휘발성 물질량을 확인할 수 있었고 이는 1,2세대 바이오 연료인 작물과 목질계에서 측정되는 값보다 높은 것으로 나타났다. 또한 원소분석한 결과 22.0 MJ kg–1의 높은 열량값이 측정되었으며 이전 바이오 및 화석 연료가 가지는 열량값보다 높거나 유사했다. 특히 Nautococcus sp. KNUA212가 가지는 열량값은 이전세대 바이오 연료인 작물과 목재에서 측정되는 값보다 높게 나타나 Nautococcus sp. KNUA212의 바이오 연료와 같은 바이오 에너지 공급원으로써의 이용 가능성도 확인할 수 있었다.

키워드
고부가가치 산물
미세조류
바이오 에너지 공급원
바이오 연료
바이오매스
지질
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Information
  • Publisher :The Korean Society of Phycology
  • Publisher(Ko) :한국조류학회
  • Journal Title :Aquatic Nature
  • Journal Title(Ko) :수생생물
  • Volume : 3
  • No :2
  • Pages :91-102
  • Received Date : 2023-11-19
  • Revised Date : 2023-12-12
  • Accepted Date : 2023-12-12
  • DOI :https://doi.org/10.23135/an.2023.3.2.3
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