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Cultivation and Biochemical Characterization of Domestic Microalga Coelastrella rubescens KNUA201 Isolated from Ulleungdo Island

울릉도에서 분리한 토착 미세조류 Coelastrella rubescens KNUA201의 배양 및 생화학적 특성 연구

Ki Yeon Koo1
,
Jeong-Mi Do12
,
Ho Sung Yoon123*
구 기연1
,
도 정미12
,
윤 호성123*
1Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu 41566, Korea
2BK21 FOUR KNU Creative BioResearch Group, School of Life Sciences, Kyungpook National University, Daegu 41566, Korea
3Advanced Bio-Resource Research Center, Kyungpook National University, Daegu 41566, Korea
1경북대학교 자연과학대학 생명과학부
2경북대학교 첨단복합 생명과학 교육연구단(생명과학부 4단계 BK교육연구단)
3경북대학교 신바이오소재연구소
*Corresponding Author
31 December 2023. pp. 77-89
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Abstract

Microalgae have characteristics of the capability of achieving rapid growth through photosynthesis and also could generate high-value products. Owing to these characteristics, microalgae are considered promising candidates in the energy application industry, particularly in the recent era of carbon neutrality. In this study, we identified indigenous sample collected from Ulleungdo Island fresh water pond as Coelastrella rubescens based on morphological feature and ITS, tufA, 18S rRNA sequences analysis from isolated strain KNUA201. 1.34 g L–1 of C. rubescens KNUA201 biomass was obtained which was grown in BG-11 media with 1~3% of CO2 for 7 days. We verified that the biomass sample, which was freeze-dried for elemental analysis after 7 days of incubation, has 25.1 MJ kg–1 of high calorific value exceeding former generation biomass such as crops and woods. Besides, mass percent of protein was 59% by biochemical analysis, α-linolenic acid (C18:3 (ω3)) accounts for 30.6% which was the highest content result from GC/MS fatty acid analysis. Based on our screening of isolated C. rubescens KNUA201 from Ulleungdo Island, we consider isolated strain have numerous usability as next generation bioenergy fuel, food additive, raw material for functional food.

Keywords
Alpha-linolenic acid
Bioenergy
Biomass
Microalgae
Ulleungdo Island

미세조류는 광합성을 통해 고속 성장을 이루며 동시에 고부가가치 산물을 생성할 수 있는 특징을 지니고 있다. 이러한 특징으로 인해 최근의 탄소중립시대에 말미암아 에너지 응용 산업에서 다양한 주목을 받고 있다. 본 연구에서는 대한민국 울릉도에서 토착 담수 미세조류 생물자원 배양주를 확보하였으며, 형태학적 및 18S rRNA, ITS, tufA 서열을 이용한 분자생물학적 동정 결과를 바탕으로 순수 분리된 KNUA201 균주가 Coelastrella rubescens임을 밝혔다. C. rubescens KNUA201 균주를 BG-11 배지에서 1~3%의 CO2를 공급하여 7일간 배양하였으며 이를 통해 1.34 g L–1의 건중량을 확보하였다. 7일 배양 후 수확한 바이오매스는 동결건조 후 원소분석을 진행하였으며 결과적으로 25.1 MJ kg–1로 확인되어 곡물계 및 목질계와 같은 이전세대 바이오매스보다 상대적으로 높은 열량값을 지니고 있음을 확인하였다. 생화학적 조성분석에서 약 59%의 높은 단백질 함량을 지닌 것으로 파악되었으며, GC/MS를 이용한 지방산 결과에서는 상업적으로 이용가능성이 높은 알파 리놀렌산(C18:3 (ω3))의 성분이 30.6%로 가장 높게 나타났다. 이러한 결과들을 토대로 울릉도에서 순수분리된 C. rubescens KNUA201 미세조류 균주는 차세대 바이오 에너지 연료, 식품 첨가물, 기능 식품 원료를 공급할 수 있는 생물자원으로서 다양한 활용 가능성에 대한 토대를 제공할 수 있을 것으로 생각된다.

키워드
미세조류
바이오매스
바이오 에너지
알파리놀렌산
울릉도
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Information
  • Publisher :The Korean Society of Phycology
  • Publisher(Ko) :한국조류학회
  • Journal Title :Aquatic Nature
  • Journal Title(Ko) :수생생물
  • Volume : 3
  • No :2
  • Pages :77-89
  • Received Date : 2023-11-10
  • Revised Date : 2023-12-05
  • Accepted Date : 2023-12-06
  • DOI :https://doi.org/10.23135/an.2023.3.2.2
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