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Original Articles

Organelle Genomes and Phylogeny of Gracilaria vermiculophylla (Gracilariaceae, Rhodophyta) from Korea

한국산 꼬시래기의 세포소기관 유전체 정보 및 계통관계

Su Yeon Kim1
,
Yeong Chae Yoo2
,
Eun Chan Yang2*
김 수연1
,
유 영채2
,
양 은찬2*
1Korea Inter-University Institute of Ocean Science and Technology, Pukyong National University, Busan 48531, Republic of Korea
2Marine Ecosystem Research Center, Korea Institute of Ocean Science and Technology, Busan 49111, Republic of Korea
1부경대학교 해양과학공동연구소
2한국해양과학기술원 해양생태연구센터
*Corresponding Author
31 December 2022. pp. 79-96
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Abstract

The Gracilariacea is a representative red algal group including many economic species. The four genera (Curdiea, Gracilaria, Gracilariopsis and Melanthalia) and 239 species were reported in the world, and two genera (Gracilaria and Gracilariopsis) and 10 species were recorded in Korea. Among them, Gracilaria vermiculophylla has been an endemic species in the northwest Pacific region, however, it also known as global invasive species since last couples of decades. This study was conducted to secure organelles genomes of Gracilaria vermiculophylla from the Goheung, Korea and to use the data for phylogenetic analysis of the species. In present study, we sequenced, assembled and annotated the plastid (ptDNA: OP978508, 180,269 bp, 29.5% GC, 238 genes) and mitochondria (mtDNA: OP978509, 26,090 bp, 28.4% GC, 52 genes) genomes of G. vermiculophylla. The ptDNA of Goheung individual showed (> 99.93% identity) similar to those of the USA and China. However, the mtDNA of Goheung was more similar to the USA (99.52% identity) than that of the China (94.16%). Total 15 distinct indels were found in mtDNAs alignment of four G. vermiculophylla (Korea 1, USA 1, China 2) individuals, and nad3-nad1-nad2-sdh4-nad4 was identified as the highly variable region in the mtDNA. The combined amino acid (AA) phylogeny based on 24 CDS (coding sequence) of mtDNA supported the close relationship of individuals between Goheung, Korea and Hog Island, USA. Newly determined ptDNA and mtDNA of G. vermiculophylla maybe useful for the diversity and phylogeny of economic red algae such as Gracilaraceae.

Keywords
Gracilaria vermiculophylla
mitochondria genome
phylogeny
plastid genome

꼬시래기과는 다세포성 진정홍조류로 유용성 종을 다수 포함하고 있다. 전세계적으로 4속(Curdiea속, Gracilaria속, Gracilariopsis속 및 Melanthalia속) 239종이 보고되었으며, 우리나라에는 2속 10종이 알려져 있다. 이 중 꼬시래기(Gracilaria vermiculophylla)는 우리나라를 포함한 북서태평양 고유종으로, 지난 30-40년간 전세계 곳곳으로 이동한 침입종으로 유명하다. 본 연구에서는 우리나라 꼬시래기의 세포소기관 유전체 자료를 확보하고 계통유연관계를 파악하고자 하였다. 전남 고흥에서 채집한 꼬시래기의 색소체 유전체(OP978508) 180,269 bp (29.5% GC, 238개 유전자) 및 미토콘드리아 유전체(OP978509) 26,090 bp (28.4% GC, 52개 유전자)를 확보하여 이전의 자료와 비교분석하고 계통수를 구축하였다. 고흥 꼬시래기의 색소체 유전체는 미국 및 중국 개체의 색소체 유전체와 99.93% 이상 유사하였다. 반면, 미토콘드리아 유전체의 경우 중국 개체(94.16%)보다 미국 개체(99.52%)와 더욱 유사하였다. 꼬시래기 4개체(고흥 1, 미국 1, 중국 2)의 미토콘드리아 유전체 정렬 결과, 15개의 삽입 및 결실 부위가 존재하였으며, nad3-nad1-nad2-sdh4-nad4 부위에서 가장 높은 변이를 보였다. 미토콘드리아 24개 CDS의 아미노산서열을 이용하여 구축한 유합계통수는 고흥 꼬시래기가 미국 Hog Island 꼬시래기와 가장 유사함을 지지한다. 꼬시래기의 새로운 색소체 유전체 및 미토콘드리아 유전체는 유용성 꼬시래기과 홍조류의 다양성 및 계통연구에 중요한 기초자료가 될 것이다.

키워드
계통분류
꼬시래기
미토콘드리아 유전체
색소체 유전체
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Information
  • Publisher :The Korean Society of Phycology
  • Publisher(Ko) :한국조류학회
  • Journal Title :Aquatic Nature
  • Journal Title(Ko) :수생생물
  • Volume : 2
  • No :2
  • Pages :79-96
  • Received Date : 2022-12-08
  • Revised Date : 2022-12-23
  • Accepted Date : 2022-12-23
  • DOI :https://doi.org/10.23135/an.2022.2.2.4
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