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Original Articles

Comparative Analysis of Two-Phased and Mixed LED Light Cultures for Enhancing Carbohydrate, Protein, and Lipid Production in Chlorella vulgaris PKVL7422
Ji Seung Han1
,
Seungjin An1
,
Woojoo Shin1
,
Tae-Jin Choi2
,
Seok Jin Oh1*
1Division of Earth and Environmental System Science, Pukyong National University, Busan 48513, Korea
2Department of Microbiology, Pukyong National University, Busan 48513, Korea
*Corresponding Author
31 December 2025. pp. 37-49
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Abstract

Microalgae, such as Chlorella vulgaris, play a crucial role in aquatic ecosystems and have substantial industrial value because of their ability to produce various useful substances, including lipids and proteins. This study aimed to determine the more efficient culture system for improving the biochemical composition of C. vulgaris PKVL7422 by comparing two-phased (TPLC) and mixed LED light culture (MLC) systems using blue (λmax=450 nm) and red (λmax=660 nm) light. In the TPLC system, the highest protein and lipid contents were observed on day 17 of the second phase under blue light, with 1.5-fold and 1.6-fold increases in protein and lipid, respectively, compared to the first phase under red light. However, no significant difference was observed in the biological compositions compared to those measured during the stationary phase under monochromatic blue light. In the MLC system, a red-to-blue light ratio of 3:7 yielded higher protein and lipid contents than the other ratios. Notably, the protein content under these conditions was 1.7 times higher than the highest content observed on day 17 of TPLC. Thus, strains such as PKVL7422 may improve the efficiency and productivity of the biological composition through the MLC method using a 3:7 (red: blue) ratio.

Keywords
Blue wavelength
Chlorella vulgaris
Mixed LED light culture
Red wavelength
Two-phased LED light culture
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Information
  • Publisher :The Korean Society of Phycology
  • Publisher(Ko) :한국조류학회
  • Journal Title :Aquatic Nature
  • Journal Title(Ko) :수생생물
  • Volume : 5
  • No :2
  • Pages :37-49
  • Received Date : 2025-12-10
  • Revised Date : 2025-12-18
  • Accepted Date : 2025-12-18
  • DOI :https://doi.org/10.23135/an.2025.5.2.3
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