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Original Articles

A Culture Study on the Spore Germination and Growth Pattern of Spongites sp.

무절산호조류 수세미말류(Spongites sp.)의 포자 발아 및 생장 패턴에 관한 배양 연구

Byung-Hee Jeon1
,
Hyung-Geun Kim2*
전 병희1
,
김 형근2*
1Korea Fisheries Resources Agency, Busan 46041, Korea
2Department of Marine Ecology and Environment, Gangneung-Wonju National University, Gangneung 25457, Korea
1한국수산자원공단
2강릉원주대학교 해양생태환경학과
*Corresponding Author
30 June 2022. pp. 21-30
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Abstract

Crustose coralline algae (CCA) attaches itself to rocky substrata and overgrows on it, has a hard body due to the deposition of calcium carbonate on their cell walls. Tetraspore of Spongites sp. was released as a sphere of 30 μm in diameter, and 24 hours later, complete attachment was achieved. Attached tetraspores differentiated in three ways; one was a germination tube growing only one direction, another was a germination tube growing in two directions, the other was germination tube which did not grow and the differentiation occurred within the attached cell. All types of the differentiations, germination occurred side part of the cell. In the early stages of the investigation, germination tube was empty and only increased sideways. After that, cytoplasm moves into the tube and filled with pink colored pigment. During the 36 days of culture, only the surface area increased, and after those 36 (culture) days, the amount of increase (increasement) of surface area decreased and discovered that cells grew vertically. In the early stages of germination, it was found that horizontal growth was performed and vertical growth was dicreased after a certain period of time.

Keywords
barren ground
Crustose coralline algae (CCA)
Culture
East sea
growth pattern

무절산호조류는 암반 기질의 표면에 얇은 두께로 부착하여 피복생장(overgrowth)을 하기 때문에 기질을 선점유하고 세포벽에 퇴적되는 방해석으로 인해 몸체가 단단한 특징을 가지고 있다. Spongites sp.의 사분포자는 직경이 약 30μm로 구형의 형태로 방출하고, 방출 후 약 24시간이 지나면서 기질에 완전히 부착하였다. 부착한 사분포자는 세가지 형태, 한쪽방향으로 발아관이 형성되는 형태와 양방향으로 발아관이 형성되어 분화가 되는 형태 그리고 발아관이 형성되지 않고 부착한 세포에서 분화가 진행되는 형태로 나타났다. 세포의 발아는 연변부에서 발생이 이루어지며, 초기에는 속이 빈 모양으로 가장자리로 크기가 증대되고 난 후 세포질 내용물이 이동 또는 생성되어 붉은 색의 색소체로 세포가 채워지는 형태로 진행되었다. 배양 36일까지는 엽면적의 증가만이 이루어졌고, 36일 이후에 엽면적의 증가량이 감소하는 대신, 세포에서 수직생장이 이루어지는 것을 확인하였다. 발아 초기에는 수평생장을 하다가 일정시기가 지나면 수직생장을 병행하는 것으로 나타났다.

키워드
갯녹음
동해안
무절산호조류
배양
생장패턴
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Information
  • Publisher :The Korean Society of Phycology
  • Publisher(Ko) :한국조류학회
  • Journal Title :Aquatic Nature
  • Journal Title(Ko) :수생생물
  • Volume : 2
  • No :1
  • Pages :21-30
  • Received Date : 2022-05-25
  • Revised Date : 2022-06-11
  • Accepted Date : 2022-06-11
  • DOI :https://doi.org/10.23135/an.2022.2.1.2
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