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Original Articles

The Effects of the Light Quality of a Light Emitting Diode (LED) on the Ammonium Uptake of Prasinophytes Tetraselmis suecica and T. tetrathele

담녹조강 Tetraselmis suecica와 Tetraselmis tetrathele의 암모니아염 흡수에 미치는 발광다이오드 파장의 영향

Kyong Ha Han1
,
Seok Jin Oh2*
한 경하1
,
오 석진2*
1Library of Marine Samples, Korea Institute of Ocean Science & Technology, Geoje 53201, Korea
2Department of Oceanography, Pukyong National University, Busan 48513, Korea
1한국해양기술원 해양시료도서관
2부경대학교 해양학과
*Corresponding Author
30 June 2022. pp. 31-38
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Abstract

Recently, it is important to find the optimal conditions for mass culture within a short period for the commercial use of food and medicine using microalgae. Thus, this study was conducted to investigate the effects of the light quality of a Light Emitting Diode (LED) on the ammonium uptake of Prasinophytes Tetraselmis suecica and Tetraselmis tetrathele. These species were exposed to a blue LED (λmax = 450 nm), a yellow LED (λmax = 590 nm), a red LED (λmax = 630 nm) and a fluorescent lamp (control) at 100 μmol/m2/s. The maximum uptake rates (ρmax) of T. suecica and T. tetrathele under the red LED were 6.35 pmol/cell/hr and 9.85 ppmol/cell/hr, respectively. The half saturation constants (Ks) of two species were 9.43 μM and 21.2 μM, respectively. The ammonium affinity of the two species under the red LED was higher than that of other wavelengths. Thus, the optimum light source to ensure economically effective and productive growth in a Tetraselmis culture system (Photo-Bioreactor) would be red LEDs because of the high growth rate shown, regardless of relatively low nutrient conditions.

Keywords
ammonium
light quality
Tetraselmis suecica
Tetraselmis tetrathele
uptake rate

최근 미세조류를 활용한 식품 및 의약품의 상업적 이용을 위해서는 단기간 내에 대량배양을 위한 최적을 조건을 찾는 것이 중요하다. 따라서 본 연구에서는 담녹조강 Tetraselmis suecica와 T. tetrathele의 암모니아염 흡수에 미치는 발광다이오드의 파장의 영향을 조사하였다. 파장은 청색 발광다이오드(λmax=450 nm), 황색 LED (λmax=590 nm), 적색 LED (λmax=630 nm) 그리고 형광램프(control)이며, 조도는 100 μmol/m2/s로 주사하였다. T. suecica와 T. tetrathele의 최대흡수속도(ρmax)는 적색 LED에서 6.35 pmol/cell/hr와 9.85 pmol/cell/hr로 나타났으며, 반포화농도(Ks)는 9.43 μM와 21.2 μM로 나타났다. 이는 적색 LED 아래에서 T. suecica와 T. tetrathele가 다른 파장보다 암모니아염에 대한 친화성이 높다는 것을 의미한다. 따라서 경제성 및 생산성 향상을 위한 Tetraselmis 배양시스템(광배양기)의 최적 광원은 낮은 영양염 상태에서도 높은 생장속도를 보이는 적색 LED가 적합할 것으로 판단된다.

키워드
암모니아염
파장
흡수속도
Tetraselmis suecica
Tetraselmis tetrathele
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Information
  • Publisher :The Korean Society of Phycology
  • Publisher(Ko) :한국조류학회
  • Journal Title :Aquatic Nature
  • Journal Title(Ko) :수생생물
  • Volume : 2
  • No :1
  • Pages :31-38
  • Received Date : 2022-05-03
  • Revised Date : 2022-06-08
  • Accepted Date : 2022-06-08
  • DOI :https://doi.org/10.23135/an.2022.2.1.3
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