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Original Articles

Effects of Light-Emitting Diodes on Photosynthetic Pigment Content and Composition of Chlorella vulgaris

발광다이오드 파장과 광량에 따른 Chlorella vulgaris의 광합성 색소 함량 및 조성 변화

Ji Seung Han1
,
Jae Hoon Noh2
,
Seok Jin Oh1*
한 지승1
,
노 재훈2
,
오 석진1*
1Division of Earth and Environmental System Science, Pukyong National University, Pusan 48513, Korea
2Marine Ecosystem and Biological Research Center, KIOST, Pusan 49111, Korea
1부경대학교 지구환경시스템과학부
2한국해양과학기술원 생태기반연구센터
*Corresponding Author
31 December 2024. pp. 63-75
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Abstract

Culturing microalgae by appropriately irradiating specific intensities and wavelengths of LED light according to the cultivation stage induces the enhancement of the synthesis of useful bioactive substances such as photosynthetic pigments and lipids. Therefore, in this study, the changes in the content and composition of photosynthetic pigments of Chlorella vulgaris were investigated according to the wavelength and light intensity of LED light. The wavelengths used were red (650nm), blue (450 nm), green (520 nm), and fluorescent lamps (control). The pigment content was highest under blue wavelength, light intensity of 100 μmol·m-2·s-1, during the stationary phase, indicating that the blue wavelength promotes the production of useful substances compared to other wavelengths. Additionally, in mixed wavelength experiments, pigments were most effectively accumulated in multi-wavelength culture where both red and blue wavelengths were irradiated simultaneously, particularly in multi-wavelength culture with a higher ratio of blue wavelength (red wavelength: blue wavelength=3:7). These results suggested that the red wavelength, which enhances high cell density, and the blue wavelength, which increases the content of useful intracellular substances, act synergistically to increase the pigment content. Therefore, using a mixed wavelength with red and blue wavelengths, especially multi-wavelength culture with a higher ratio of blue wavelength, contributes to economic efficiency and increased productivity.

Keywords
Chlorella vulgaris
LED
photosynthetic pigment
wavelength

발광다이오드의 특정 광량과 파장을 적절하게 조사하여 미세조류를 배양한다면, 광합성 색소와 같은 유용 생리활성물질의 합성을 증진할 수 있다. 본 연구에서는 발광다이오드 파장과 광량에 따른 Chlorella vulgaris의 광합성 색소 함량 및 조성의 변화를 조사하였다. 파장은 적색 파장(650 nm), 청색 파장(450 nm), 녹색 파장(520 nm) 그리고 형광램프(control)을 주사하였다. 색소의 함량은 청색 파장, 광량 100 μmol·m-2·s-1, 정상기에서 높게 나타났기 때문에 다른 파장에 비해서 청색 파장이 유용물질을 증진시키는 작용을 하는 것으로 보인다. 또한 혼합 파장 실험 중 적색 파장과 청색 파장을 동시에 조사하는 다중 파장 배양에서 색소가 가장 효과적으로 축적되었으며, 청색 파장의 비가 높은 다중 파장 배양(적색파장:청색파장=3:7)에서 함량이 높았다. 이러한 결과는 높은 세포밀도를 얻을 수 있는 적색 파장과 세포 내 유용물질의 함량을 증진시키는 청색 파장이 상승효과로 작용하여, 색소의 함량이 증가한 것으로 생각된다. 따라서 청색 파장과 적색 파장을 혼합한 혼합 파장 중에서도 청색 파장의 비가 높은 다중 파장 배양을 이용한다면, 경제적인 효과 및 생산성 증대에 기여할 수 있을 것으로 판단된다.

키워드
광합성 색소
발광다이오드
파장
Chlorella vulgaris
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Information
  • Publisher :The Korean Society of Phycology
  • Publisher(Ko) :한국조류학회
  • Journal Title :Aquatic Nature
  • Journal Title(Ko) :수생생물
  • Volume : 4
  • No :2
  • Pages :63-75
  • Received Date : 2024-12-16
  • Revised Date : 2024-12-24
  • Accepted Date : 2024-12-24
  • DOI :https://doi.org/10.23135/an.2024.4.2.3
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